Protocols

 

C-terminal tagging Drosophila (2016/04/06)

N-terminal tagging Drosophila (2016/04/06)

C-terminal tagging mammalian cells (2015/02/26)

 

The protocols are "work in progress", it is a good idea to check from time to time if an updated version has been posted.

Some of the reagents are currently unpublished. Please contact Klaus Förstemann if you would like to obtain unpublished material - we are happy to share it unless there is a conflict with our own work.

Please cite these article as a reference for our work:

Kunzelmann et al, G3 2016; doi1: 10.1534/g3.116.028241

A comprehensive toolbox for genome editing in cultured Drosophila cells

Pubmed-link

Böttcher et al., Nucleic Acids Res. 2014;42(11):e89. doi: 10.1093/nar/gku289.

Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells.

Pubmed-link